Establishment of animal model of osteoarthritis and selection.docVIP

 PAGE \* MERGEFORMAT 37 Establishment of animal model of osteoarthritis and selection Author: Di Ji-liang, Weng Xi-sheng, Qiu-Xing Keywords: animal model of osteoarthritis and selection Osteoarthritis (osteoarthritis, OA) is a degeneration of articular cartilage, articular margin of the main changes in bone hyperplasia disease, the clinical cure is still a lack of measures to establish animal models to study the pathogenesis of OA, treatment and prevention of the necessary means. OA sub-secondary and primary, so OA animal model can be used from production methods are divided into two categories: one for the induced model, that is, through a variety of methods, such as joint brake operations, surgery, intra-articular injection of substances inducing OA production; another spontaneous model, that is, without any outside intervention, animals spontaneously OA, such as the C57 black mice, STR / ort mice, etc.; In addition to these two types of models, there is also an analog modeling method Osteoarthritis that the articular cartilage cells in vitro. 1-induced model of 1.1 Joint Brake Braking can be classified according to their position of flexion, the middle bit, straight bit, straight bit fashion which may be imposed by external compression; the joints of animals long-term fixed at a certain location, due to joint stress can produce changes in articular cartilage back variable and thus the formation of OA. Flexion due to reduced contraction of the muscles across the knee joint articular cartilage caused by atrophic changes; In terms of straight-bit brake, limiting the movement of joints, muscles, joint capsule contraction, on the articular surface of the excessive pressure produced , leading to the occurrence of OA 〔1〕; straight bit and exert pressure on the joint surface due to increasing pressure to further promote the formation of OA. Evans et al 〔2〕 observed that the rabbit knee joint brake 60 d,