Establishment of rat vestibular organ culture model and observed under the action of phorbol ester ultrastructural changes.docVIP

 PAGE \* MERGEFORMAT 11 Establishment of rat vestibular organ culture model and observed under the action of phorbol ester ultrastructural changes [Abstract] Objective: To establish a rat vestibular organ culture model of the original generation of in vitro and, through TEM vestibular epithelial cells in phorbol ester (PMA) before and after the ultrastructural changes. Methods: Under sterile conditions, remove the 2 d of Health rat utricular epithelium, in vitro 1 ~ 7 d; started from the first 2 inverted microscope observation, photography, experimental group joined PMA 30 min, 2 h and 6 h, fixed, transmission electron microscopy observation. Results: In vitro cultured oval capsule 1 wk, 12 h when fibroblasts can be seen along the edge of swim graft, 2 d after the endothelial cell aggregation, showing paving stone-like, TEM cells, no swelling, membrane, nucleus integrity of mitochondria without swelling, cilia No shedding; PMA morphologically normal cells in the test group, ribosome-intensive, compared with the previous well-developed Golgi apparatus, endoplasmic reticulum expanded slightly. Conclusion: The rat’s vestibular organ culture method is feasible for the future provide an experimental model for further study. [Keywords:] hair cells; vestibular; organ culture techniques; phorbol esters In the modern medical and biological research has been widely applied in tissue culture technology, it has the in vivo studies can not match advantage. In vitro model of in vivo studies to overcome the problem can not be resolved, the application technology can expand the inner ear in vitro culture scope of the study, in order to study the mechanisms and treatment of deafness cytology provides a model system, and to in vitro under the conditions of the inner ear pathology, physiology and pharmacology in-depth research can be carried out [1-3]. We are in the establishment of the vestibular organ culture in vitro Based on the model, using transmission electron microscop