Experience denaturing polyacrylamide gel.docVIP

 PAGE \* MERGEFORMAT 5 Experience denaturing polyacrylamide gel [Keywords:] denaturing polyacrylamide gel electrophoresis separation of the vertical, preparation experience Denaturing polyacrylamide gel electrophoresis is commonly used techniques of molecular biology, one of the irrigation system than the standard gel electrophoresis gel tank irrigation system difficulties, bubble gum and often leakage is inevitable, be it through repeated practice to master skills in irrigation system. We mRNA differential display technique in screening with adult nephrotic patients with steroid sensitive nephrotic syndrome gene differences between the experiments, the use of denaturing polyacrylamide gel electrophoresis separation of PCR product bands, silver nitrate staining into the filter bar differences between the two genes. now with the relevant reference [1-2] reported on the gel following the experience. 1 sheet ready (1 sheet of clean plastic irrigation to the success of the first critical step. Repeated use of glass and the interval between when the chip to remove the gel completely and thoroughly cleaned and dry. With ethanol wipe residual water stains on the glass. there must be no residual gel and the residue, or plastic irrigation will produce bubbles. (2 Glue on the glass before processing, respectively. long glass with pro-and silane, silane treated short glass with peel, easy to electrophoresis after the length of the separation of glass, the gel is adhering to a long glass. silane treatment applied first with a short strip of glass, and then pro-and long-silane coated glass, can not change gloves to avoid contamination pro-and silane short glass. Glass board can not have excess silane, or the watermark will stay dry in the follow-up experiments such as silver nitrate staining bands there will be a watermark traces of color bands observed. can wipe the excess silane lens paper. smearing uniformly separated drying glass, the best drying overnight,