Extract of Tripterygium early separation and identification of terpenoid.docVIP

 PAGE \* MERGEFORMAT 12 Extract of Tripterygium early separation and identification of terpenoid [Abstract] Objective: To find a large, fast, stable and isolated and purified from Tripterygium bioactive substances. Methods: explore a range of solvent system, in TBE 300A high-speed countercurrent chromatography, the crude extract from Tripterygium diterpenoid compound isolated and purified and used 1H NMR and 13C NMR identification of compounds. Results: crude extract from Tripterygium purified five diterpenoids were Triptonide, different mine new lactone phenol , Hutch Su, Lei Lei phenol phenol terpene lactones and ether, purity 98.2%, 96.6%, 98.1%, 95.1%, 96.5%. Conclusion: The method of high-speed countercurrent chromatography, the extract from Tripterygium step 5 isolated diterpenoid for diterpenoid triptolide compounds laid the foundation for further study. [Keywords:] Tripterygium wilfordii; diterpenoids; high-speed countercurrent chromatography [Abstract] Objective: To find an efficient and convenient way to separate bioactive compounds from Tripterygium wilfordii. Methods: A possible most suitable solvent system was investigated and a high capacity counter current chromatography instrument was tried to separate the diterpenoids from Tripterygium wilfordii. Results: Five diterpenoids were separated from Tripterygium wilfordii and these diterpenoids were identified as triptonide, isoneotriptophenolide, hypolide, triptophenolide and triptonoterpene methyl ether. Conclusions: High capacity counter current chromatography is a simple, repeatable and convenient way to separate bioactive compounds from Tripterygium wilfordii. [Keywords:] Tripterygium wilfordii; Diterpenoids; High speed counter current chromatography TWH is a famous traditional Chinese medicine, 200 years of widely used to treat a variety of clinical disease [1,2]. In recent years, it was discovered that ethanol extract of Tripterygium diterpenoid compounds in vivo and in vitro exp