Extraction and purification of polysaccharide Schisandra Optimization.docVIP

 PAGE \* MERGEFORMAT 15 Extraction and purification of polysaccharide Schisandra Optimization Of: Jiang Bo, Sun Jing, Liu Chun, FAN Sheng [Abstract] Objective To study the parameters of Polysaccharide extraction and parameter evaluation. Methods of water extraction and alcohol precipitation Polysaccharide was extracted by single factor and orthogonal experiment to study the solid-liquid ratio, extraction temperature, extraction time and extraction times on the Polysaccharide yield was studied, and the rate of polysaccharide content of quality assessment and evaluation methods to explore the simultaneous purification of polysaccharides. Results Polysaccharide extraction temperature on the yield of the largest solid-liquid ratio, extraction time Polysaccharide yield of less influence. polysaccharide content by measuring the extraction yield calculated with the simple Polysaccharide with polysaccharide (calculated from sediment yield compared Polysaccharide there are some differences, indicating that the determination of the method used mass ratio than the simple conditions established more accurately. Conclusion Polysaccharide optimum conditions of extraction: solid-liquid ratio 1:30, temperature 90 , extraction time 4 h, extracted 3 times. Sevage method using activated carbon and joint use of purified polysaccharide, the purity of the polysaccharides extracted from the 43.48% to 66.43% increase. [Keywords:] Extraction and Purification of Polysaccharide Schisandra Schisandra is the fruit of Magnolia, its sweet, sour, acrid, bitter, salty flavors and taste, so named Schisandra. Schisandra is a precious traditional Chinese medicine, “lt;Compendium of Materia Medicagt;gt; recorded, Schisandra benefits gas indication, Keni on the gas, injured workers Leishou, to fill the gap, strong negative, raising the five internal organs, in addition to heat, vomiting inverse, tonic labor, eyesight, strong bones, Chufan heat, drug hangover, only fluid thirst, lack of