Extraction of effective parts of Corydalis.docVIP

 PAGE \* MERGEFORMAT 12 Extraction of effective parts of Corydalis 【Abstract】 Objective: To improve the extraction of total alkaloids Corydalis, Corydalis alkaloids to find the best extraction process. Methods: The orthogonal design method, in order to Tetrahydropalmatine for the detection of targets, using high performance liquid chromatography Tetrahydropalmatine content. Results: The pH of the Tetrahydropalmatine yield a very significant impact. Conclusion: The use of alkaline methanol extract the best. Keywords: dl; Tetrahydropalmatine; HPLC; extraction process; Determination Corydalis Corydalisy anhusuo WTWang. Has the blood circulation of qi analgesic effect, the main active ingredients of alkaloids, of which Corydalis A, B and ugly elements such as the main [1]. Corydalis A, B and ugly elements such as a better analgesic effect, to the role of the strongest elements B [2], the total alkaloid with anti-ulcer, inhibit gastric acid secretion, antispasmodic and to increase coronary blood flow, anti-arrhythmia and other effects [3]. Control the quality of Corydalis, and many more Tetrahydropalmatine as the preferred ingredient. In this paper, orthogonal experimental design, by high performance liquid chromatography (HPLC) determination of Corydalis extract the content of tetrahydropalmatine to select the optimum conditions of extraction Corydalis. An instrument and reagent Japan’s Shimadzu Corporation LC  6A high performance liquid chromatography, C  R6A data processing device, Japan’s Shimadzu Corporation AUE  210 electronic balance (1 / 10000); the reagents were HPLC pure water for the home-made ultra-pure water; dl B prime reference substance (Chinese medicines and biological products), Corydalis medicine provided by the Tianjin Chinese Herbal Pieces plants. 2 Methods and Results 2.1 Orthogonal experimental method preferred by Ethanol Corydalis Corydalis alkaloids according to the physical and chemica