Extraction of onion polysaccharide extraction of different research.docVIP

 PAGE \* MERGEFORMAT 13 Extraction of onion polysaccharide extraction of different research Author: Zhang Feng Lun Wu Suling Sun Xiaoming Zhang Weiming [Abstract] Objective To compare the different extraction methods on the extraction of polysaccharide onion. Methods thermal flooding formulation, microwave-assisted freeze-thaw method and ultrasonic method from fresh onions and onion-skin extract polysaccharides, polysaccharide content measured by DNS method. The results using glucose as standard, using DNS Determination of polysaccharide is feasible, with an average recovery rate of 98.5%, RSD is 2.65%. Whether it is fresh fleshy part of the onion or onion powder, microwave-assisted extraction of freeze-thaw method was the highest rate of polysaccharides and ultrasonic extraction of the highest content of polysaccharide, in which freezing and thawing by microwave-assisted extraction of polysaccharides yield of onion skin in 3.58% ( content of 63.9%); fleshy part of the polysaccharide yield was 3.15% (content 62.0%). Conclusion Microwave-assisted Extraction of freeze-thaw method is more appropriate method of polysaccharide onion, the onion-polysaccharides extracted a high rate, and the polysaccharide content is higher. [Keywords:] onion-polysaccharides of microwave ultrasonic Abstract: ObjectiveTo compare the effects of different extraction methods on extraction of the polysaccharides from onion. MethodsPolysaccharides was extracted from fresh onion and onionskin by hot water leaching method, microwave-assisted freeze thawing method and ultrasonic wave method. The content of polysaccharides was determined by DNS method. ResultsUnder the condition that the glucose was confirmed as standard, it was accurate and feasible to determine the content of polysaccharides in onion by DNS method; the average recovery rate reached 98.5% with RSD of 2.65%. Weather fresh onion or onionskin dry powder, the yield of polysaccharides obtained by microwave-assisted freeze thawi