Extraction of Ganoderma lucidum.docVIP

 PAGE \* MERGEFORMAT 6 Extraction of Ganoderma lucidum Author: Ma Minghui, Lv Lijuan, Gong Ji-Yu, Zhao Wei [Abstract] Objective: To examine indicators for the extraction of polysaccharides to improve the extraction of Ganoderma lucidum. Methods: boiling liquid PH value, the first time, boiling time, boiling time than, plus liquid volume four factors, each taking three levels. Results: The analysis of variance results showed that the A 3 B2 C 2 D 1 is the best extraction process, namely, boiling liquid PH value of 11, the first time, boiling time of 120min, boiling time, the ratio of 3.0:2.0:0.5, add fluid volume to 8 times. Conclusion: The greatest impact the first time, boiling time, boiling time, and processing fluid volume also has a significant impact, boiling liquid PH value is minimal impact. [Keywords:] Ganoderma lucidum; polysaccharide; extraction process; orthogonal test Pharmacological experiments show that Ganoderma lucidum polysaccharide immune regulatory function, enable the hepatitis B surface antigen negative conversion [1]. Production are mostly concentrated decoction extract, a low yield, in order to improve the yield, seeking the best extraction process, this experiment has been studied for its extraction process [2-3]. 1 Materials and methods 1.1 Materials Ganoderma lucidum, Jilin Province Wang seized medicine company, TG328B electronic analytical balance, UV-1810 UV - visible spectrophotometer, analytical balance, R205 rotary evaporator, the reagents used were of analytical grade. 1.2 Methods 1.2.1 Optimization of extraction process orthogonal design based on traditional extraction method and the physicochemical properties of polysaccharides, consider boiling liquid PH value (factor A), the first time, boiling time (factor B), boiling time than (Factor C) , plus liquid volume (factor D) 4 factors, each factor set up three levels, in order to extract the amount of polysaccharide inspection indicators, orthogonal test. Table 1.