Extraction of fat-soluble part of Salvia cold soak Technology.docVIP

 PAGE \* MERGEFORMAT 5 Extraction of fat-soluble part of Salvia cold soak Technology Author: Lin Ze, Liu Donghui, Guan Shi-Xia [Abstract] [Objective] To investigate the cold soak method of fat-soluble parts of Salvia extract the optimum conditions. [Methods] high performance liquid chromatography (HPLC) method for the determination of methods to extract the amount of tanshinone Ⅱ A targets, effects of different concentrations of ethanol and smash right tanshinone Ⅱ A rate of extraction and used for further optimization orthogonal extraction conditions. [Results] The optimum extraction condition for the introduction of eight times the amount of the volume fraction of 95% ethanol, cold soak extracted two times (each time 3 h), in such conditions, the availability of tanshinone Ⅱ A higher rate of extraction. [Conclusion] This process conditions a simple, stable, high extraction rate. [Keywords:] Salvia / production and preparation; Salvia / chemistry; tanshinone Ⅱ A / analysis; chromatography, high pressure liquid; orthogonal test Labiatae Salvia Plant Salvia is Salvia miltiorrhiza Bge.’s Dried root and rhizome, with blood stasis and pain, promoting blood circulation, the efficacy of pure heart Chufan [1]. Modern medicine, Salvia has the expansion of coronary artery, increase coronary blood flow, prevention of myocardial ischemia and myocardial infarction, accelerate microcirculation, promote capillary network and opening up, inhibit myocardial contraction, reduce energy consumption, expand blood vessels, reduce cholesterol, blood lipids, inhibit blood coagulation, fibrinolysis activation, and stability of erythrocyte membrane to enhance hypoxia tolerance and increase blood flow to the liver, protect liver damage cells and anti-hepatic fibrosis, inhibit the central nervous system, as well as anti-inflammatory antibacterial effects [2]. Its fat-soluble ingredient has anti-bacterial, anti-inflammatory, antioxidant and other pharmacological effects [3]. Salvi