Extraction of cough mixture Xuanfei.docVIP

 PAGE \* MERGEFORMAT 8 Extraction of cough mixture Xuanfei Of: Ma Guoping, Stanley Lau, Qian Fang, Lu Chao [Abstract] Objective To optimize the extraction process Xuanfei cough mixture. Methods Ephedrine hydrochloride, n-butanol extract yield and water extraction rate as the index, using orthogonal test method to study water extraction process conditions: amount of water, extraction time and extraction times. best extraction results were: 8 times the amount of water, boiling 2 times, each time 60 min. Conclusions The results of this study, reliable, optimal conditions for mass production. [Keywords:] Xuanfei cough mixture, ephedrine hydrochloride, orthogonal, extraction process Abstract: Objective To optimize the extraction process of Xuanfei Zhike Mixture. Methods The preparation process was investigated by L9 (34) orthogonal design with content of ephedrine hydrochloride, the extraction rate of n-butanol and total extraction as indexes. The main factors influencing the extraction efficiency such as the amount of added water, the time of extraction and times of boiling were investigated. Results The optimum technical condition was as follows: 8 times amount of water, extract 2 times and 60 min for each time. Conclusion The result is reliable. It can be used for mass production. Keywords:: Xuanfei Zhike Mixture, ephedrine hydrochloride, orthogonal test, extraction process Xuanfei cough mixture from the ephedra, bellflower, licorice root, Polygala, Morus alba, fried Arctiin etc., with Xuanfei Liyan, moist cough efficacy, clinical use cough, allergic cough and on respiratory infections, itchy cough after a cold and so on. The orthogonal experimental design to determine the optimum extraction process. An instrument and reagent HPLC Agilent 1100, quaternary pump (G1311A), oven (G1316A), VWD detector (G1314A), workstation (Chemstation), Agilent manual injector (10 μL loop) .1/10 million electronic balance (BP-211D type, Germany Sartorius),