Extraction and Purification of Quyou lotion Research.docVIP

 PAGE \* MERGEFORMAT 3 Extraction and Purification of Quyou lotion Research Author: Ju Jian-Ming Qian, SHEN Hong-wei ZHU Ling-ying Lee [Abstract]: Objective To study Quyou lotion preparation process, optimization optimal extraction and purification process conditions. Methods HSYA A content, solid content for the detection of targets, using orthogonal experiment investigated three kinds of factors (plus water, boiling time, boiling times) the impact of its water-boiling process; and then compared Different concentrations of alcohol to the mixed effect of Shen. Results Quyou lotion the best conditions for water extraction process 12 times the amount of added water, boiling 2 times, 1 h / time; to hybrid technology with better than 60% alcohol precipitation. Preparation of a reasonable conclusion. [Keywords:] high-performance liquid chromatography Quyou lotion orthogonal alcohol precipitation hydroxysafflor Yellow-A Quyou lotion is of Jiangsu Province Chinese Medicine Research Institute clinical experience side effects exactly. That party from Euphorbia, safflower, Banlangen composed of 6 Herbs with heat-clearing and detoxification, insecticide dehumidification of power, for the treatment of various wart. Safflower based mainly Medicine Smell Fang, the main active ingredient HSYA A. The preparation process in order to ensure the reasonableness, we HSYA A study as the main indicators of water boiling on the prescription process and purification process was optimized to determine a reasonable preparation process. An instrument and reagent 1.1 Instrument Waters high performance liquid chromatography (Alliance2695 quaternary pump and automatic sampling systems, 996 diode array detector, Empower chromatography workstation); Millipore Milli-Q Water Purification System; METTLER one ten-thousandth and one millionth electronic balance. 1.2 reagent HSYA A reference substance (Lot No. :11637-200502), by China’s pharmaceutical and biological products to provide for