Fang Zhou Ke Kim Yan A549 lung cancer cell resistance to the analysis of polysaccharides.docVIP

 PAGE \* MERGEFORMAT 11 Fang Zhou Ke Kim Yan A549 lung cancer cell resistance to the analysis of polysaccharides Of: the United States Yang Ping, Yin Lin, Zhang Xu, Hao, Sun Yi, Duan Jin Ao [Abstract] Objective Zhou Ke Kim Yan material foundation for the Treatment of lung cancer. Methods extraction and alcohol precipitation Fang Zhou Ke Kim Yan polysaccharide purified by Sevag, DEAE cellulose ion exchange resin preparation polysaccharides, UV detection protein retention situation, IR group ownership of herbs, the use of anthrone - sulfuric acid Determination of polysaccharide content. results in the effective polysaccharide at 240 nm and 260 nm no absorption, the infrared spectrum and the group of herbs Jade bamboo, and Agrimony Maozhaocao infrared spectra are very similar, Fang Zhou Ke Kim Yan to the polysaccharide content of 1.41%. Conclusion polysaccharide against A549 lung cancer cells without protein and nucleic acid, source of odoratum herbs in the group, and Agrimony Maozhaocao, Fang Zhou Ke Kim Yan a certain amount of polysaccharides. [Keywords:] Fang Zhou Ke Kim Yan, polysaccharides, anti-cancer activity in A549 cells Abstract: Objective To study content and ascription of the composition of the polysaccharides from Zhou’s Prescription for Resolving Mass. Method The polysaccharide was extracted by ethanol precipitation, and purified by savage method. DEAE-cellulose anion exchange resin was used to prepare the composition of polysaccharides . UV-spectra was used to detect protein and IR-spectra was used to analyses the ascription of the composition of polysaccharides. The content of the composition of polysaccharides was determined by anthranone-sulfuric acid method. Result There was no absorption at 240 nm and 260 nm. The IR spectrum graph of the polysaccharides was similar to Polygonatum odoratum, Radix Ranunculi Ternati and Agrimonia pilosa Ledeb. The content of the composition of polysaccharides was 1.41%. Conclusion The composition of