Fetal growth restriction and normal rat offspring hippocampus compared proteome map of two-dimensional electrophoresis.docVIP

 PAGE \* MERGEFORMAT 16 Fetal growth restriction and normal rat offspring hippocampus compared proteome map of two-dimensional electrophoresis Of: yellow spectrum, Gou Wen Li, M. Yang, Zhang Rui, Sun Yunping [Abstract] Objective To compare the fetal growth restriction (FGR) and normal rat hippocampus in rat offspring dimensional gel electrophoresis proteome maps of different proteomics technology in the FGR to explore the nervous system caused by abnormal development of offspring study the possibility of applying . Methods SD rats of FGR model of isolated rat offspring to organize and extract total protein, the two-dimensional electrophoresis to compare FGR offspring rats with normal protein expression in hippocampus. The results of rat offspring and FGR normal rat hippocampus were detected two-dimensional electrophoresis map of 647 and 626 protein spots on the two matches after electrophoresis of 18 protein spots were found only in the hippocampus of rats with FGR-DE protein expression profiles, 28 protein In group 2 in the hippocampus occurred 3 times more content changes, including increased FGR group of 25, down 3. Conclusion initially established animal model of FGR method for comparative proteomics technology, FGR protein in hippocampus of rats group significantly different from normal rats, the difference point for further research found that FGR offspring of higher nervous activity caused by abnormalities in the pathogenesis provide useful clues. [Keywords:] fetal growth restriction, hippocampus, learning and memory, two-dimensional electrophoresis ABSTRACT: Objective To compare the global protein patterns between the hippocampus of fetal growth restriction (FGR) offspring rats and control rats hippocampus by means of two dimensional electrophoresis (2 DE) so as to explore the possibility of applying proteomics technology in research on FGR induced developmental abnormality of the offspring s nervous system. Methods FGR model of Sprague Daw