For the establishment of two cell lines in vitro comparison of fatty liver model.docVIP

 PAGE \* MERGEFORMAT 18 For the establishment of two cell lines in vitro comparison of fatty liver model Study: Pan Xuefeng, Wencai Xia, Xu Jianhua [Abstract] Objective To compare the oleic acid induced hepatocellular carcinoma cell line HepG2, normal liver cell line L02 established model of liver steatosis, by examining the preventive effect of choline methionine compound, looking for simple, stable and in vitro drug screening model. Methods The normal group, HepG2 model group, HepG2 different concentrations of treatment group, L02 model group, L02 different concentrations of treatment group, with oleic acid induced liver steatosis, methionine choline compound to intervene to oil red O staining fat cells were observed drop formation conditions and to detect cell supernatants ALT, AST, GT level. The results of oleic acid stimulation HepG2, L02 liver cells 24 h, the typical fat droplets within cells. methionine choline compound 2 dose, drug difficult to make HepG2 cells decreased the lipid droplets, L02 cells in 48 h, Suan fuel oil red O staining Zhengming intracellular lipid droplets can be reduced, the expression of lipid droplets in red IOD values, the treatment group compared with the model group, the difference was significant ( P lt;0.01). supernatant ALT, AST, GT, methionine choline compound in each group compared with the model group were significantly different, confirming its small liver cell injury. Conclusions L02 is more suitable than the in vitro HepG2 drug screening cell models, the formation of fatty acid stimulation of L02 cells change, ahead of 24 h of drug intervention, 24 h after administration of 1, modeling and administration of programs that can be used as a viable model of fatty liver cells using the drug screening . [Keywords:] hepatoma cell line HepG2; L02 liver cell line; oleic acid; fatty liver; model; vitro; Compound methionine choline Abstract: Objective To compare two models of hepatocytic steatosis in vitro, which we