Formoterol in asthmatic airway goblet cell hyperplasia and mucin MUC5ac mRNA expression.docVIP

 PAGE \* MERGEFORMAT 17 Formoterol in asthmatic airway goblet cell hyperplasia and mucin MUC5ac mRNA expression Of: Zhang Wei, Tan Yanfang, country *, Chen Ming, Liang Rui Yun, Lv Zhiqiang [Abstract] [Objective] To investigate the long-acting 2 agonist formoterol on asthma (bronchial asthma), airway goblet cell hyperplasia and mucin MUC5ac mRNA expression. [Method] 40 female SPF level BABL / c mice were divided into 4 groups of 10 each. A group for the saline control group; B group, egg protein (ovalbumin, OVA) asthma; C group intervention for asthma formoterol group; D group asthma dexamethasone group. In the last challenge, mice were taken 24 h after the left lung tissue of all periodic acid Schiff (PAS) staining, determined by medical image analysis software goblet cell area of #8203;#8203;the bronchial epithelial cells in the percentage of the total area and calculate the number of goblet cells. get right lung tissue real-time qRT-PCR, detection of mucin MUC5ac mRNA expression. [results] B group of mice the number of goblet cells of bronchial epithelium, goblet cells accounted for a total area of #8203;#8203;epithelial cells percentage and MUC5ac mRNA levels were significantly higher than that of A group [(163.63 + - 16.68) months vs (0.46 + - 0.16) a, (77.36 + - 5.05)% vs (0.03 + - 0.01)%, (10.31 + - 0.73) vs (1.00 + - 0.13), P all lt;0.05]; C group of mice the number of goblet cells of bronchial epithelium, goblet cells accounted for a percentage of the total area of #8203;#8203;epithelial cells and MUC5ac mRNA levels were lower than the B group [(52.04 + - 4.60) a vs (163.63 + - 16.68) months, (30.05 + - 3.72)% vs (77.36 + - 5.05)%, (1.64 + - 0.14) vs (10.31 + - 0.73), P all lt;0.05]; D Group mice the number of goblet cells of bronchial epithelium, goblet cells accounted for a percentage of the total area of #8203;#8203;epithelial cells and MUC5ac mRNA levels were lower than the B group [(63.41 + - 6.39) a vs (163.63 + - 16.68) months, (38.52 + - 3