Fragile X syndrome on prenatal screening and diagnosis.docVIP

 PAGE \* MERGEFORMAT 11 Fragile X syndrome on prenatal screening and diagnosis OF: OF ADULT EDUCATION OF Fang Yi Jia Zhang Aidong Yang Dantong [Abstract Objective fragile X syndrome, prenatal genetic screening and diagnosis. Methods Using polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis in 32 cases of pregnant women and their fetus’s fragile X gene (CGG ) n repeat sequences were detected by PCR amplification of both genes on sex baby teeth were identified. Results in 32 cases of pregnant women and their fetuses, pregnant women were detected in 1 case before the mutation carriers, and 1 case of fragile X male fetus comprehensive Zheng patients. Conclusion PCR amplification of fragile X gene (CGG) n repeats, combined with gene amplification of sex identification of immature teeth, fragile X syndrome can be conducted on prenatal screening and diagnosis. [Keywords:] Fragile X syndrome; polymerase chain reaction; teeth immature gene; prenatal diagnosis Prenatal screening and diagnosis for fragile X syndrome YANG Dantong, JIA Yifang, WU Aihua, et al. Shandong Provincial Key Laboratory for Improving Birth Outcome Technique Shandong Provincial Family Planning Science and Technology Institute, Jinan, Shandong 250002, China [Abstract] Objective To screen and diagnose fragile X syndrome prenatally by PCR. Methods The CGG repeat region in the FMR 1 gene was amplified by PCR and was detected by PAGE in 32 pregnant women and their fetuses and the fetal sex was identified by the amplification of amelogenin gene. Results One premutation carrier and one male fetus with fragile X syndrome were detected from 32 pregnant women and their fetuses. Conclusion Amplification of the CGG repeat region in the FMR 1 gene and the amelogenin gene by PCR can be applied for prenatal screening and diagnosis for fragile X syndrome. [Keywords:] Fragile X syndrome; Amelogenin gene; Prenatal screening; Prenatal diagnosis Fragile X syndrome (fragile X syndr