Freeze-dried human red blood cells before the optimization study of the load trehalose.docVIP

 PAGE \* MERGEFORMAT 28 Freeze-dried human red blood cells before the optimization study of the load trehalose Author: Zhuang Yuan Jing-Han Liu Feng-Chen Lin Xi-Lin Ouyang Car Series 【Abstract】 In order to better implementation of trehalose in the freeze-dried red blood cells stored in the protective effect, the key is to overcome the non-permeability of the plasma membrane of trehalose to achieve an effective concentration of trehalose in the cytoplasm. The purpose of this study of human red blood cells by the regularity of the load trehalose, trehalose loading screen out the best load conditions and to evaluate the trehalose loading of red blood cells of various physical and chemical indicators. At different incubation temperatures (4,22 and 37 ℃), incubation time (0,2,4,6,8,10 hours), different load buffer concentration (0,200,400,600,800,1 000 mmol / L) of fresh red blood cells under the conditions of testing the success of the Trehalose loading red blood cell volume and the physical and chemical indicators; at a fixed load conditions, of fresh red blood cells and red blood cells stored at 4 ℃ for 72 hours trehalose load, free hemoglobin (FHb), hemoglobin (Hb ), and mean corpuscular volume (MCV) were compared. The results showed: red blood cells of the trehalose load and incubation temperature, time and the load buffer is closely related to the concentration of trehalose. As the temperature increased, the time extension and the load buffer, the increase in trehalose concentration, red blood cell uptake of trehalose is also increased. Under the best conditions in the trehalose loading, fresh red blood cells and red blood cells stored at 4 ℃ for 72 hours the concentration of intracellular trehalose, FHb concentrations were 65.505 ± 6.314 mmol / L, 66.2 ± 5.002 mmol / L and 6.567 ± 2.568 g / L, 16.168 ± 3.922 g / L. Conclusion: The best conditions for red blood cell load trehalose is used fresh red blood cells, at 37 ℃ conditions, trehalose concen