Freeze-drying experimental study of platelet preservation.docVIP

 PAGE \* MERGEFORMAT 9 Freeze-drying experimental study of platelet preservation Authors: Jing-Han Liu, Jun Zhou, Wang Dongmei, Xi-Lin Ouyang, Xing Yan-chao, Lu Fa-Qiang Abstract This study investigated the method of freeze-dried platelet preservation in order to access to long-term preservation of freeze-dried platelet products, so that it can at room temperature under the conditions of preservation, small footprint, light weight, easy to transport over long distances to meet unexpected events and the need for treatment of war wounds. Saved in the freeze-drying process of adding reversible platelet activation inhibitors, DMSO and trehalose and other low-temperature protective agent to carry out pre-processing, refrigeration, a dry, two drying, rehydration, and simultaneous determination of platelet recovery, thrombin aggregation response, promoting coagulation, CD62p expression and PAC  1 expression rate. The results showed: platelet recovery was 56.29%, its accumulation of thrombin response and the control group no significant difference in pairs of ADP and n-propyl gallate-induced aggregation of responses than the control group decreased 49.34% and 26.25%, promoting coagulation and the control group and no statistical difference; freeze-dried platelet CD62p expression rate of 42.36%, PAC  1 expression rate was 2.12%, thrombin activated CD62p re-expression rate was 50.88%, PAC  1 re-expression rate was 54.55%. Conclusion: Adding reversible platelet activation inhibitors, trehalose and DMSO after the freeze-dried platelet aggregation and procoagulant activity of platelet function and fresh no significant difference in platelet activating reversible inhibitors reduce the expression of freeze-dried platelet CD62p increase the viability of freeze-dried platelets, thus prolonging their survival time, thus the basis of the freeze-drying method for preservation of freeze-dried platelets to further improve efficiency. Keywords: reversible a