Four Mongolian children Engrg -1 crude extract on rat normal liver cell line proliferation BRL.docVIP

 PAGE \* MERGEFORMAT 9 Four Mongolian children Engrg -1 crude extract on rat normal liver cell line proliferation BRL Of: Zhoucheng Jiang Zhou and Yan Ling Yan-Fang Yang Club [Abstract] Objective: To observe the Mongolian children Engrg -1 (TGLG-1 crude extracts of four normal rat liver cells BRL Proliferation. Methods: Preparation of a Mongolian child Engrg -1 of ethanol, butyl alcohol, ethyl acetate, petroleum ether extract, normal rat liver cell line BRL, Mongolian children observed by MTT colorimetric Engrg -1 extract on rat liver cells in vitro toxicity of the dose-effect relationship. Results : Mongolian children Engrg -1 four extracts on liver cell proliferation have shown as having a minimum inhibitory concentration, ethanol extract was 0.3 mg / mL, n-butanol extract of 0.1 mg / mL, ethyl acetate and petroleum ether extracts are all 1 10-2mg/mL. in the minimum inhibitory concentration, crude extracts of the four inhibitory rate compared with the control group, no significant difference (P lt;0.05. at least drug concentration of 1 10-3mg/mL and between each of the minimum inhibitory concentration, each drug has emerged as the drug concentration increased the inhibition rate decreased. and in each of the minimum inhibitory concentration and maximum drug concentration of 0.5 mg / mL, between each drug has emerged as the drug concentration increased, the trend of higher cell inhibition rate. Conclusion: Four Mongolian children Engrg -1 crude extract on rat liver cell line proliferation show BRL promote the proliferation of low-dose, high dose inhibition phase characterized by dose - effect relationship. [Keywords:] Mongolian children Engrg -1, rat liver cells, MTT assay Mongolian children Engrg -1 is a single taste of Mongolian preparations shown in clinical lipid-lowering, anti-inflammatory effect. The main components of Artemisia Asteraceae leaf, stem parts, a wide distribution in China, especially in Inner Mongolia has rich resources. throu