Freeze-drying protective agent on the active components of HB liposome encapsulation efficiency and particle size of.docVIP

 PAGE \* MERGEFORMAT 20 Freeze-drying protective agent on the active components of HB liposome encapsulation efficiency and particle size of Author: Juan Hu Shui-Gen BIAN Jun Wang Guohua Ke heaven and earth 【Abstract】 Objective To study the different kinds and concentrations of freeze-dried protective agent against HB before and after the effective components of freeze-dried liposome encapsulation rate and the average particle size effects. Method prescribed by 16 active components of HB was prepared liposomes, using fluorescence spectrophotometry encapsulation rate, with a microscope and digital image analysis software determined the average particle size, before and after the adoption of freeze-dried liposome encapsulation rate and Comparison of the optimized size of the freeze-drying protective agent. The results of the best protective agent for the freeze-dried mannitol and binary protective agent sugar - glycine, the encapsulation rate of 69.91% and 66.70%, appearance good effect, the ratio, respectively, mannitol: PC: CH: HB = 4:1 : 0.5:0.2; sucrose: glycine: PC: CH: HB = 4:0.8:1:0.5:0.2. Conclusion protective agent can reduce the freeze-dried liposome vesicle at the freeze-drying process of the damage as well as liposome encapsulation efficiency and particle size effects. Keywords: liposome entrapment efficiency of freeze-dried protective agent particle size Abstract: ObjectiveTo study the effects of freeze-drying on the endcapsulation and vesicles size of HB liposome with different kinds of lyoprotectents at various concentrations.MethodsSixteen formulations of HB liposome were designed.The entrapment efficiency was examined by fluorospectrophotometry.The particle size of the preparation was examined by microscope and digital imaging analysis software.The optimized lyoprotectent was selected based on the comparison of entrapment efficiency and particle size before and after freeze-drying.ResultsThe use of sucrose as a primar