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Fresh frozen plasma coagulation factors and analysis of test results
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Fresh frozen plasma coagulation factors and analysis of test results
[Keywords:] of fresh frozen plasma; coagulation factor; activity
According to the Ministry of Health technical specifications of clinical blood transfusion, fresh frozen plasma (fresh frozen plasma, FFP) after collection from the blood within 6 ~ 8 h are separated into the -50 frozen into blocks, stored 1 year at -30 contains almost all coagulation factors, including the instability factor and [1 2]. The vulnerability of the blood plasma clotting factor preparation, storage and transport conditions, the effects of inactivation, leading to reduced efficacy of blood. but because FFP is still the clinical prevention and treatment out coagulopathy of blood products, so to understand and grasp the blood clotting factors in FFP in the content and activity is extremely important. We offer our hospital blood bank for the city’s FFP random sampling 10, the part of the clotting factor inspection, the results reported below.
1 Materials and methods
1.1 The sampling sources of material 10 are from the Bengbu city FFP Blood, ACD A prepared from anticoagulated whole blood. Experimental equipment: TECO COATRON 1800 automated blood coagulation analyzer. Reagents are produced DADE BEH RING . standard serum (Lot.No.102518): PT reagent (Lot.No.102627): APTT (Lot.No.103128): Fib reagent (Lot.No.102511); TT reagent (Lot.No.101465); F (Lot.No.103821), F (Lot.No.100708), F (Lot.No.100181).
1.2 10 randomly selected sampling FFP, 37 water bath melts, immediately after thawing of plasma collected by aseptic 2 ml, then on the automatic determination of coagulation factor coagulation analyzer. Clotting factor determination in strict accordance with CO 1800 automatic coagulation analyzer operating instructions and reagents required, the use of condensing optical detection principle of the different test items using different dilutions of the standard setting time of plasma coa
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