Glucosamine sulfate on IL-1β -induced synthesis of human osteoarthritis chondrocytes IL-1 Ⅱ receptor and the effects of IL-1Ra.docVIP

 PAGE \* MERGEFORMAT 5 Glucosamine sulfate on IL-1β -induced synthesis of human osteoarthritis chondrocytes IL-1 Ⅱ receptor and the effects of IL-1Ra Wang Xiaobin, Wong Kung Yee, Xue Qingyun, SUN Chang-tai [Abstract] Objective To study the glucosamine sulfate (glucosamine sulfate, GS) on IL-1β -induced cultured human osteoarthritis chondrocytes synthesized type Ⅱ HOC interleukin-1 receptor (interleukin - 1 receptor type Ⅱ , IL-1R Ⅱ ) and interleukin-1 receptor antibody (interleukin-1 receptor antagonist, IL-1Ra) effects. Methods 10 cases of osteoarthritis patients undergoing total knee arthroplasty of the femoral condyle and tibial plateau cartilage specimens, enzyme digestion method to obtain cartilage cells in vitro. In the original and second generation of human cartilage cells in the culture medium by adding IL-1β (5ng/ml) and different concentrations of GS (2mmol / L, 10mmol / L, 20mmol / L) the role of 24h, application of ELISA assay GS right IL -1β -induced HOC synthesis of interleukin-1 receptor antibody, applied RT-PCR detection of type Ⅱ HOC interleukin-1 receptor (interleukin-1 receptor type Ⅱ , IL-1R Ⅱ ) of the mRNA. Results IL-1β stimulated IL-1Ra synthesis of cartilage cells increased (P amp;lt;0.01), synthesis of IL-1R Ⅱ mRNA decreased (P amp;lt;0.05). Compared with the control, 2mmol / L and 20mmol / L, GS can inhibit IL-1β stimulated chondrocytes expressing IL-1R Ⅱ mRNA reduction (P amp;lt;0.01), and that this modification works in a concentration-dependent manner (P amp;lt;0.01 ), and 0.2mmol / L, GS does not affect IL-1β reduced IL-1R Ⅱ mRNA expression (Pamp;gt; 0.05). Used alone 20mmol / L of GS on cartilage cells IL-1R Ⅱ mRNA had no effect (Pamp;gt; 0.05). 0.2mmol / L, 2mmol / L and 20mmol / L of GS on IL-1β stimulated IL-1Ra synthesis of cartilage cells had no effect (Pamp;gt; 0.05). Conclusion glucosamine sulfate antagonized by IL-1β synthesis in human osteoarthritis, IL-1R Ⅱ cells down-regulate, but does not affect the expr