Green June HepG2.2.15 cells with serum on inhibition of HBV cccDNA.docVIP

 PAGE \* MERGEFORMAT 9 Green June HepG2.2.15 cells with serum on inhibition of HBV cccDNA Of: Huang Fang, Lin Xing, Huang Renbin, Zhang Shijun [Abstract] Objective To observe the June Green (Liuyueqing, LYQ) in vitro anti-hepatitis B virus (HBV) effect. Methods Serum pharmacological method, in vitro HBV cell culture system (2.2.15 cells) were LYQ anti-HBV cccDNA role of observation. Results LYQ HepG2.2.15 containing serum in cell culture can effectively inhibit replication of HBV cccDNA, the effect was obvious dose-response relationship and time. Conclusion LYQ significantly inhibit in vitro the role of hepatitis B virus . [Keywords:] June Green; hepatitis B virus; HepG2.2.15 cells; HBV cccDNA Abstract: ObjectiveTo study the anti-HBV activity of Liuyueqing (LYQ) in vitro. MethodsThe HBV in vitro cell culture system (HepG2.2.15 cell) was used for examining the anti-HBV activity of LYQ with Serum-pharmacology. ResultsThe serum containing LYQ could decrease the HBV DNA level in HepG2. 2. 15 cells, and its inhibitory effects were dose-and time-dependent. ConclusionLYQ can inhibit HBV DNA in vitro significantly. Keywords:: Liuyueqing; HBV; HepG2.2.15 cell; HBV cccDNA June Green (Liuyueqing, LYQ) Department of Acanthaceae (Acanthaceae) plant Xiao cages (top flower Malan) Taraphochlamys affinis (Gr iff) Bremekhu [Strobilanthes affinis (Griff) YCTang] part of the dry ground [1]. Our group preliminary study found that young drug-containing serum on June HepG2.2.15 cells was significantly inhibited HBsAg-HBeAg [2], the total saponin on HBV-DNA replication was significantly inhibited [3]. This study was June Green HepG2.2.15 cells with serum on the role of HBV cccDNA, in June to further explore the mechanism of anti-hepatitis B Green. 1 Materials and equipment 1.1 Drugs, June Green (Liuyueqing, LYQ) collected from Guangxi Mountain, one from the First Affiliated Hospital of Guangxi Medical pharmacists identified Fang Huang, the water extract from th