High-performance liquid chromatography in different areas of Ningxia wolfberry rutin and chlorogenic acid content of.docVIP

 PAGE \* MERGEFORMAT 16 High-performance liquid chromatography in different areas of Ningxia wolfberry rutin and chlorogenic acid content of Author: Zhang Zi-Ping, Liao-ling, SHI Xiao-Wen 【Abstract】 Objective To establish a RP-HPLC method for simultaneous determination of rutin in Ningxia wolfberry and chlorogenic acid method. Method of chromatographic analysis of the conditions Shim-pack VP  ODS column (250 mm × 4. 6 mm, 5 μm), acetonitrile   water-acetic acid (5:94.5:0.5) as mobile phase A, acetonitrile: water: acetic acid (70:29.5:0.5) as mobile phase B, using gradient elution, flow rate 0.5 ml * min-1, detection wavelength 342 nm, column temperature was 30 ℃. The results of chlorogenic acid and rutin were linear in the range 10 ~ 100 μg * ml-1 (correlation coefficient r = 0.999 5, respectively, and r = 0.999 8), the average recovery (n = 5) were 98.6%, 99.0 %; different origin, different years, Ningxia wolfberry samples of rutin and chlorogenic acid content is different. Conclusion This method is good reproducibility and is suitable for simultaneous determination of rutin in Chinese wolfberry and chlorogenic acid content. Keywords: Ningxia wolfberry; flavonoids; high-performance liquid chromatography; rutin; chlorogenic acid Abstract: ObjectiveTo develop an RP  HPLC method with gradient dilution for simultaneous determination of chlorogenic acid and rutin in Lycium barbarum L. from different habitats.MethodsThe separation was performed on a Shim-pack VP  ODS 250 mm × 4.6 mm (5 μm) , acetonitrile / water / acetic acid (5:94.5:0.5) was used as the mobile phase A and acetonitrile / water / acetic acid (70:29.5:0.5) as the mobile phase B with gradient elution, the flow rate was 0.5ml * min-1, the detection wavelength was at 342 nm and the temperature was 30 ℃. ResultsThe good linearities of chlorogenic acid and rutin were obtained over the ranges of 10 ~ 100 μg * ml-1 (r = 0.999 5 and r = 0.999 8 respectively), the average recov