High-performance liquid chromatography method for simultaneous determination of Rutin in Eucommia leaf herbs such as three kinds of flavonoid content of active ingredient.docVIP

 PAGE \* MERGEFORMAT 12 High-performance liquid chromatography method for simultaneous determination of Rutin in Eucommia leaf herbs such as three kinds of flavonoid content of active ingredient Author: Ng Hing Wang Xingning when Caogui Hong Zhang [Abstract] Objective To develop a simultaneous determination of Eucommia leaf rutin, quercetin, kaempferol high-performance liquid chromatography. Methods Spherisorb BDS C18 column (5 μ m, 250 mm * 4.6 mm), with methanol -0.1% phosphoric acid solution with gradient elution (methanol: 0 min, 30%; 10 min, 30%; 30 min, 50%; 40 min , 60%; 50 min, 30%), flow rate 1 ml * min-1, detection wavelength 350 nm. The results of samples rutin, quercetin, kaempferol recoveries were 96% ~ 98%, 93% ~ 97%, 96% ~ 101%; rutin, quercetin, kaempferol line sex in the range of 0.579 ~ 5.79 μ g, r = 0.999 9; 0.12 ~ 1.20 μ g, r = 0.999 8; 0.109 ~ 1.09 μ g, r = 0.999 9. Conclusion Eucommia leaf herbs from different areas of Guizhou in rutin, quercetin and kaempferol levels vary greatly. [Keywords:] high-performance liquid chromatography Eucommia leaf rutin quercetin kaempferol Eucommia Eucommia ulmoides Oliv belongs to the State two protected plants is the deciduous genus Eucommia Caudoux Chung tree species endemic to China, the Department of the geological history of the Quaternary glacial movement left over from the relict species accounted for health [1,2]. A supplement liver and kidney, strengthening the bones, the efficacy of tocolysis, primarily for the treatment of kidney back pain, weak bones, pregnancy endoleak, fetal movement anxiety, hypertension [3], whose skin is a rare endemic to China, nourishing medicine, modern research Eucommia leaves and found that skin contain the same chemical composition, has the same pharmacological effects, Eucommia leaves health care products is also an important variety of valuable raw materials. Eucommia medicinal flavonoid compounds are one of the main active ingredient with a variety of biologi