HPLC determination of capsaicin in Capsicum and dihydro capsaicin content.docVIP

 PAGE \* MERGEFORMAT 7 HPLC determination of capsaicin in Capsicum and dihydro capsaicin content [Abstract] Objective To establish a determination of Capsicum in two main components, and dihydro capsaicin pepper Determination of alkali content. Methods using high performance liquid chromatography, C18 column (4.6 mm × 250 mm, 5 μm), methanol - water (70:30) as mobile phase, flow rate 1.0 mL / min, detection wavelength was 280 nm. the results of capsaicin and dihydro capsaicin at 1.955 ~ 7.820 μg (r = 0.999 9), 2.709 ~ 7.224 μg (r = 0.995 5) was linear within the range of average recoveries were 101.57%, 108.87%. Conclusion The method is simple, accurate, reliable, and can be used in the determination of Capsicum and dihydro capsaicin content of capsaicin . [Keywords:] Capsicum, Capsaicin, dihydro capsaicin, high performance liquid chromatography, determination Abstract: Objective To establish an HPLC method to determine the two principal constituent - capsaicin and dihydrocapsaicin in general alkaloids of Capsaicin. Method The analysis was performed on C18 column (4.6 mm × 250 mm, 5 μm), with methanol-water (70: 30) as the mobile phase. The flow rate was 1.0 mL / min and the detection wavelength was set at 280 nm. Result The linear relationships of capsaicin and dihydrocapsaicin were within the range of 1.955 ~ 7.820 μg (r = 0.999 9) and 2.709 ~ 7.224 μg (r = 0.995 5), respectively. The average recoveries were 101.57% and 108.87%, respectively. Conclusion The method is convenient, the result is accurate and reliable, and can be used to determine the content of two principal constituent in general alkaloids of Capsaicin. Keywords:: general alkaloids of Capsaicin, capsaicin, dihydrocapsaicin, HPLC, content determination Capsicum pepper plants in the methods provided by the physical training, refining, often used as analgesic, anti-inflammatory, blood circulation, frostbite, itching, sterilization, detoxification, rheumatism, pain, weight loss. Ca