HPLC Determination of chlorogenic acid in Yinhuang Tablets content of.docVIP

 PAGE \* MERGEFORMAT 6 HPLC Determination of chlorogenic acid in Yinhuang Tablets content of [Abstract] Objective: To establish a Silver Yellow Tablets chlorogenic acid content determination. Methods: 18 alkyl bonded silica gel as the stationary phase, methanol - water - acetic acid - triethylamine (15:95:1:0.3) as mobile phase, detection wavelength of 324nm, external standard method. Results: chlorogenic acid into the sample volume of 0.0824 ~ 0.824μ g between, showing a good linear relationship, r = 0.9999, average recovery was 99.39%, RSD of 0.71%. Conclusion: HPLC determination of chlorogenic acid in Yinhuang Tablets content fast, simple, accurate and reliable. [Keywords:] high performance liquid chromatography (HPLC); silver yellow tablet; chlorogenic acid Silver pornographic films from honeysuckle, Scutellaria baicalensis extract made preparations processed with heat-clearing and detoxifying, anti-inflammatory of power, mainly for the treatment of respiratory tract infections, pharyngitis embolism. In this paper, HPLC Determination of Silver Yellow Tablets honeysuckle the main component of chlorogenic acid content in order to monitor its content. An instrument and reagent Shimadzu LC-10A-based high-performance liquid chromatography; SPA-10 UV detector; chromatography workstation. Chlorogenic acid reference substance: China Biological Products, Lot :0753-200212; silver yellow tablets, factory pre-production, batch number: 20.05091 million. Chromatography pure methanol, acetic acid, triethylamine were chemically pure. 2 Methods and Results 2.1 Chromatographic conditions and system suitability test column was Z00BAX (4.6mm * 250mm); mobile phase: methanol - water - acetic acid - triethylamine (15:85:1:0.3); detection wavelength 324nm; column temperature at room temperature ; flow: 1ml.min; theoretical plate number by chlorogenic acid of dollars should not be less than 3500. 2.2 Preparation of solution for the test materials obtained in this study