HPLC Determination of Compound Rhubarb Oral Ornidazole membrane of emodin.docVIP

 PAGE \* MERGEFORMAT 9 HPLC Determination of Compound Rhubarb Oral Ornidazole membrane of emodin Author: Wang Jin Nan, SHAO Chang-jiang, HE Hall, Jia [Abstract] Objective To develop a high performance liquid chromatography (HPLC) Determination of Compound Rhubarb Oral Ornidazole membrane emodin. Methods HPLC, column was Agilent ZORBAXSB-C18 (4.6mm * 150mm, 5μ m), mobile phase was methanol -0.1% phosphoric acid solution (4:1), flow rate 1.0ml/min, column temperature 30 ℃ , detection wavelength of 254nm. The results of emodin in the 0.04 ~ 0.4μ g/ml (r = 0.9998) the scope of a good linear relationship. Emodin average recoveries were 98.25%. Conclusion The method is simple, rapid and accurate. [Keywords:] compound rhubarb Ornidazole oral membrane; Emodin; high-performance liquid chromatography [Abstract] Objective To establish a method for the determination of emodin in compound ornidazole-rhubarb buccal film by HPLC.Methods A HPLC method was used with Agilent ZORBAXSB-C18 column (150 mm * 4.6mm, 5μ m). The mobile phase was methanol - 0.1% phosphoric acid (4:1). The flow rate was 1.0mL/min. The column temperature was 40 ℃ and the detection wavelength was 254nm. Results The calibration curve was linear in the range of 0.04 ~ 0.4μ g (r = 0.9998). The average recovery was 98.25%. Conclusion The method is simple, rapid and accurate. [Keywords:] compound ornidazole-rhubarb buccal film; emodin; HPLC Periodontitis is a common and frequently-occurring disease, infections caused by anaerobic bacteria, the local application of antibiotics for the treatment of periodontal disease have a good effect. Previously reported periodontitis film with multi-biological non-degradable materials for the accessories in use require regular replacement. Compound Rhubarb Oral Ornidazole film from the School of Pharmacy, Lanzhou University and Lanzhou Municipal First People’s Hospital Dental cooperative development agreement as a physician’s prescription to treat periodontitis, curati