HPLC Determination of Lovastatin in Monascus fermentation broth contents.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of Lovastatin in Monascus fermentation broth contents [Abstract] Objective To determine the Monascus fermentation broth Lovastatin concentration method. Methods HPLC method using methanol as solvent, vibration shaker (150 r / min) 3 h extraction of lovastatin, chromatographic conditions were: methanol  18 mmol / L phosphoric acid solution (volume ratio 80:20) as mobile phase, The flow rate was 1.0 mL / min, detection wavelength was 237 nm. The results of lovastatin at the 5 ~ 50 μ g / mL concentration and peak area within the linear relationship was good, plus sample average recovery was 100.75%, RSD 0.87%. Conclusion The method is simple and effective, reproducible and can be used as Monascus-related products, quality control. [Keywords:] high-performance liquid chromatography; Monascus; lovastatin Abstract: Objective To establish an HPLC method for determining the content of lovastatin in fermentation broth of Monascus. Method Lovastatin could be completely extracted from the fermentation broth by methanol under shaking for 3 h with 150 r / min. Then, samples were analyzed on the HPLC column using methanol  18 mmol / L phasphate (80:20) as mobile phase under the flow rate of 1.0 mL / min. The detection wavelength was 237 nm. Result The method had good linearity over the concentration of 5 ~ 50 μ g / mL for lovastatin. The average recovery was 100.75% and RSD 0.87%. Conclusion The method is convenient, effective and accurate, which is suitable for the quality control of Monascus products. Keywords:: HPLC; Monascus; lovastatin Monascus applications in China for nearly two thousand years of history, the early study of Monascus focused on their production of Monascus pigment and a variety of enzymes such as amylase, glucose amylase, glucoamylase, protease, pectin enzymes, it is mainly used for the early Monascus food coloring, wine, food fermentation [1,2]. 1979 Japanese scholars Endo [3] from the culture of Mona