HPLC Determination of Hesperidin spleen fertility tablets.docVIP

 PAGE \* MERGEFORMAT 10 HPLC Determination of Hesperidin spleen fertility tablets [Abstract] Objective To establish the spleen fertility determination of hesperidin tablets. Methods HPLC, column: KromasilC18 column (4.6 mm 250 mm, 5 m), mobile phase was methanol acetic acid water (volume ratio 35: four sixty-one), column temperature at room temperature, flow rate was 1.0 mL min-1, the detection wavelength was 283 nm. Results hesperidin 0.300 8 ~ 2.105 6 g range and peak area was linear (r = 0.999 9 , n = 7), the average recovery was 101.68%, RSD 1.45%. Conclusion The method is accurate, reproducible, and can be used for fattening the quality control spleen. [Keywords:] spleen fertility tablets; hesperidin; HPLC method Abstract: Objective To establish a determination method for hesperidins in Jianpizengfei tablets by HPLC. Methods HPLC was performed on a Kromasil C18 column (250 mm 4.6 mm, 5 m) with Methanol acetic acid Water (35:4:61) as the mobile phase at a flow rate of 1 mL min-1 and the detection wavelength was at 283 nm.Results It showed a good linearity at the range of 0.300 8 ~ 2.105 6 g (r = 0.999 9, n = 7) and the average recovery of hesperidins was 101.68% (RSD = 1.45%). Conclusion The method is sensitive, accurate, reproducible, specific and can be used for the quality control of Jianpizengfei tablets efficiently. Keywords:: Jianpizengfei tablets; hesperidins; HPLC Fertility tablets from the spleen of Atractylodes, Astragalus, Citrus and other Shisan Wei medicine made with energy and the spleen, soothe the nerves of the effect, mainly used for appetite sluggish, stunted growth, weight loss, restless sleep and so on. Atractylodes reuse this side King and medicine, astragalus qi solid form, antiperspirant detoxification, Citrus spleen qi, tune in, dampness, a total of Fangzhong Chen medicine. Citrus Citrus containing element, hesperidin, hesperidin and other new components, including orange Paper glycosides has strong biological acti