HPLC determination of puerarin safflower and hawthorn extract mixture of puerarin and HSYA A content of.docVIP

 PAGE \* MERGEFORMAT 8 HPLC determination of puerarin safflower and hawthorn extract mixture of puerarin and HSYA A content of Author: Lee Bridge, Song Tian, Wang Guangzhong, Liu Yan-Wen [Abstract] Objective To establish a RP-HPLC method, Determination of puerarin, hawthorn, safflower extract mixture of puerarin and HSYA A content. Methods Hypersil ODS 4.6 mm (i.. D.) * 250 mm, 5μ m, with 0.2% (V / V) phosphoric acid solution: methanol: acetonitrile (81:16:3) as mobile phase, flow rate 1.2 ml / min, column temperature 25 ℃ , detection wavelength were 250,403 nm, measured puerarin and HSYA A. The results of puerarin and HSYA A linear in the range of 0.032 406 ~ 0.162 08 mg / ml and 0.029 4 ~ 0.147 mg / ml; average recoveries were 98.3%, 98.0%; RSD were 1.2% , 1.6%. Conclusion The method is fast, simple, accurate, and can evaluate the quality of Extracts provide a basis. [Keywords:] puerarin HSYA A high performance liquid chromatography Abstract: Objective: To establish an RP-HPLC method for the determination of the content of Puerarin and HSYA in the extracts from Radix Puerariae, Fructus Crataegi and Carthamus tintorius.MethodsThe HPLC system consisted of Hypersil ODS column (250 mm * 4.6 mm, id 5 μ m) with phosphoric acid (0.2%): methanol: acetonitrile (81:16:3) mixture as mobile phase, the flow rate was 1.2 ml / min, the detection wavelength was 250,403 nm respectively for determination of Puerarin and HSYA , and the column temperature was 25 ℃ . ResultsThe linearity ranges of Puerarin and HYSA were in the range of 0.032 406 ~ 0.162 08mg/ml and 0.0294 ~ 0.147mg/ml respectively, the average recoveries of adding sample were 98.3% and 98.0%, the RSD (n = 5) were 1.2% and 1.6% respectively. ConclusionThe method is simple, fast and accurate. It can be used for the quality control of the extracts of Radix Puerariae, Fructus Crataegi and Carthamus tintorius. Keywords:: Puerarin; HSYA; HPLC Naodesheng side is ‘the Chinese Pharmacopoeia’ classical side [1], from