HPLC determination of radiation on the flowers Swertia gentiopicroside contents.docVIP

 PAGE \* MERGEFORMAT 7 HPLC determination of radiation on the flowers Swertia gentiopicroside contents Of: Xiu Bridge, Huang Fengjiao, Chen Jiachun, Liu Yan Wen [Abstract] Objective To establish the convergence flowers Swertia method of determination of bitter glycosides. Methods HPLC column was Alltimal C18 (5 μm, 250 mm × 4.6 mm), mobile phase was methanol - water (water containing 0.05% phosphoric acid), gradient elution, flow rate 1 mL / min, detection wavelength 234 nm, column temperature was 30 ℃. gentiopicroside Swertia results in the range 3.10 ~ 30.98 μg good linear relationship, the correlation coefficient was 0.999 1, the average recovery was 98.0%, RSD = 2.66% (n = 6). Conclusion The method is simple, separation is good, its quality standards for the establishment of an experimental basis. [Keywords:] spoke flowers, high performance liquid chromatography, Swertia bitter glycosides, determination Abstract: Objective To establish a method for content determination of swertiamarin in Lomatogoniopsis alpina. Methods HPLC method for determination was used. Chromatographic column: Alltimal C18 (250 mm × 4.6 mm, 5 μm). Mobile phase: methanol-water (including 0.05% H3PO4), and gradient elution. Flow rate: 1 mL / min. Wavelength: 234 nm. Column temperture: 30 ℃. Results The calibration curve of swertiamarin was in good linearity over the range of 3.10 ~ 30.98 (r = 0.999 1). The average recoveries were 98.0%, with RSD = 2.66% (n = 6). Conclusion It is a simple and sensitive method in controlling the quality of Lomatogoniopsis alpina. Keywords:: Lomatogoniopsis alpina, HPLC; swertiamarin, determination Gentianaceae convergence convergence spent flowers from plants Lomatogoniopsis alpina TNHo et SWLiu, mainly in northeastern Tibet, Qinghai and Sichuan, the south, was born in spruce forest edge, meadows and shrub meadow slope. Convergence flower is the State Council in August 4, 1999 approved the “national key protected wild plants (first batc