HPLC determination of rutin content in different areas Coltsfoot Flower.docVIP

 PAGE \* MERGEFORMAT 11 HPLC determination of rutin content in different areas Coltsfoot Flower 【Abstract】 Objective To study the Wuxi contained in different places of the Coltsfoot rutin differences. Method of HPLC determination of rutin content of butterbur. Mobile phase: methanol  water (0.025 ml / L) phosphoric acid (40:60); detection wavelength of 360 nm. The results of the standard curve was Y = 6 934.5X-336 5 (r = 0.999 9), rutin at 0.040 08 ~ 0.400 8 μg range of linear relationship; the average recovery was 99.40%, RSD of 2.14%. Conclusion The method is simple, fast and accurate; Wuxi different places of the Coltsfoot Flower of rutin concentration close to suitable for large-scale cultivation in the local. Keywords: high performance liquid chromatography rutin Wuxi County Coltsfoot Flower Abstract: ObjectiveTo determine rutin in buds of Tussilago farfara L. from different areas. MethodsThe content of rutin was determined by HPLC with methanol-water (0.025 mol * ml-1) phosphorous acid (40:60) as mobile phase, detective wavelength was 360 nm.ResultsThe standard curve for rutin was Y = 6 934.5X-3 365 (r = 0.999 9), linear range was 0.040 08 ~ 0.400 8 μg, the average extraction recovery was 99.40%. ConclusionThis method is simple, sensitive and rapid, and the contents of rutin in T. farfara from different areas are similar. Tussilago farfara L. is suitable to be planted in Chongqing Wuxi county. Keywords:: Tussilago farfara L.; Chongqing Wuxi county; HPLC; Rutin Coltsfoot Flower for the Asteraceae genus Tussilago farfara Tussilago farfara Tussilago farfara L. Bud. For the common cough and asthma drugs, mainly contains flavonoids, sesquiterpenes, alkaloids and volatile oil [1]. Coltsfoot Flower of the research literature, mostly pharmacological and chemical aspects, Wuxi constituents of different origin has not been reported. Coltsfoot Flower Wuxi County, Chongqing is the traditional place of origin, have a long histo