HPLC determination of the content of quercetin in hawthorn fruits.docVIP

 PAGE \* MERGEFORMAT 9 HPLC determination of the content of quercetin in hawthorn fruits [Abstract] Objective To establish the HPLC determination of quercetin content in Hawthorn method. Methods chromatographic conditions: Shim-Pack VP-ODSC18 column, mobile phase was methanol -0.4% phosphoric acid (52:48, V / V), detection wavelength was 373 nm. The results of the regression equation of quercetin Y = 2 855.4X-10 974 (r = 0.999 9, n = 5), the linear range of 0.018 9 ~ 0.056 6μ g; the average recovery was 96.44%, RSD 1.51%. Conclusion This method is simple and reliable for the quality control of herbs hawthorn provides fast and accurate determination method. [Keywords:] Hawthorn High Performance Liquid Chromatography Determination of quercetin Abstract: ObjectiveTo develop a method for determination of the content of quercetin in Crataegus sanguinea by HPLC.MethodsThe separation was carried out on Shim-Pack VP-ODS C18 column with methanol-0.04% phosphoric acid solution (52:48) as mobile phase, the detection wavelength was 373nm. ResultsThe regression equation and coefficient was Y = 2 855.4X-10 974 (r = 0.999 9, n = 5) for quercetin. The linear range was 0.018 9 ~ 0.056 6μ g. The average recovery and precision (RSD ) of quercetin was 96.44% and 1.51%, respectively. ConclusionThis method is simple, accurate and can be used for the quality control of Chinese Hawthorn. Keywords:: Crataegus sanguinea; Quercetin; HPLC; Quantitative determination Hawthorn is commonly used traditional Chinese medicine, with Xiaoshi stomach and the effectiveness of Qi bulk deposition, the main ingredients of flavonoids and organic acid compounds [1]. Hawthorn can be blood pressure, blood fat, increase coronary blood flow, cardiac and anti-arrhythmic, the active ingredient for the flavonoids [2], whose main components are the fruits of quercetin and hyperin [3]. At present, the ‘Chinese Pharmacopoeia’ is only determined by its organic acids (as citric acid dollars) of the content o