Human Assisted Reproductive Technology in the embryo culture and selection.docVIP

 PAGE \* MERGEFORMAT 16 Human Assisted Reproductive Technology in the embryo culture and selection Of: Wangbing Song, Yuan Shui Kiu, Hu Shumin, Liu Xinyu, Zhou Yu Yan, Sun Lei Xu, Liu Ying [Keywords:] Shenyang, Liaoning, Shenyang 204 Hospital, Research Center for Reproductive Medicine With the expanding field of reproductive biology, human assisted reproduction has been the rapid development of appropriate, since the world’s first in vitro fertilization - embryo transfer (IVF) since the birth of the baby [1], the technology has been carried out about three years. However, while IVF has become the treatment of infertility improve the program, its success rate is still low. Most of the embryos died before transplantation seems to occur in the training phase, only 50% of the embryos in the sixth day in vitro developed to the blastocyst stage [2], transplantation of embryonic development as the proportion of fetuses less than 15%. To improve the pregnancy rate, with implantation of multiple embryos, but resulted in a significant increase in multiple pregnancy rate. Therefore, how to improve culture conditions to optimize gamete quality and embryonic development of new screening technology to improve the implantation rate has been assisted reproduction laboratory researchers focus and direction. An in vitro embryo In the past ten years, people have realized that by optimizing the culture medium can improve the in vitro development of IVF embryos and embryo implantation rates. By reducing the concentration of glucose in the medium, adding amino acids and growth factors can increase the rate of blastocyst formation and cell number. in the improvement of IVF embryos on the medium composition has been little research space. It is reported that even with glucose-free medium to improve embryo quality, pregnancy rate has not increased [3]. Human embryos during preimplantation development, nutritional needs change as follows: early cleavage mainly for the