Hypoxic preconditioning of mouse hippocampus the expression of Fas-L.docVIP

 PAGE \* MERGEFORMAT 10 Hypoxic preconditioning of mouse hippocampus the expression of Fas-L Of: the original tree Jiang Zhang Sheng Zhou Gao Lijun Shao Country Club [Abstract] Objective: To investigate the reproducibility of tissue hypoxia on the hippocampal expression of Fas-L protein. Methods: Hypoxia 0 (H0), 1 times (H1), 4 times (H4) to take the hippocampus after organization, application Western Blot technique to detect within the hippocampus of mice with the protein expression of Fas-L. Results: It was found that H0, H1 and H4 group within the hippocampus to express Fas-L, H4 H0 group did not differ between groups, H1 were significantly higher (P lt;0.05, compared with H0 and H1. Conclusion: Acute hypoxic preconditioning of mice Fas-L expression in the hippocampus change. [Keywords:] Hypoxic preconditioning; hippocampus; Fas-L Abstract Objective: To detect the effect of hypoxia preconditioning on Fas-L expression in acute repeated hypoxic mice hippocampus. Methods: Mice were divided into 3 groups randomly and were exposed to hypoxia for 4 runs (H4 group), 1 run (H1 group) and 0 run (control group), respectively. Western was used to examine the Fas-L level in mice hippocampus after exposure to hypoxia, with total protein extracted from mice hippocampus for Western blot. Results: Fas-L was found in H0, H1 and H4 group. Fas-L protein levels were found to be increased in H1 (P lt;0.05, vs.H4 and H0). There is no difference between H4 and H0. Conclusion: The change of Fas-L protein level is closely related to protection of the brain and the formation of preconditioning in hypoxic preconditioned mice. Keywords: Hypoxic preconditioning; Hippocampus; Fas-L Hypoxic / ischemic brain damage is caused by the most common clinical factors that seriously affect human health. Although brain hypoxia / ischemia is extremely sensitive, but the nerve cells against hypoxia / ischemia have a certain tolerance. 1990, Kitagawa et al [1] found that there is bra