Hydroquinone bone marrow stromal cells in vitro expression of transcription factors.docVIP

 PAGE \* MERGEFORMAT 11 Hydroquinone bone marrow stromal cells in vitro expression of transcription factors Authors: YANG Haiyu, Yang Jianguo, Wang Guanghan, Kang - 【Abstract】 In order to study hydroquinone (hydroquinone, HQ) on cell activator, phorbol ester (PMA) caused by bone marrow stromal cells (BMSC) nuclear transcription factor expression, and to explore with the relationship between the hematological toxicity of benzene. In vitro culture method to obtain bone marrow stromal cells, to observe the morphological changes; were used to NF-κB P65 by immunohistochemical methods and kits TransAM P65 measured with different concentrations of hydroquinone plus activator PMA stimulation of bone marrow stromal cells, NF - κB protein expression and activity dynamics. The results showed that: in each group after the cell with HQ, as compared with the control group, P65 protein from the nucleus back to the cytoplasm surrounding the nucleus, staining was weakly positive; different concentrations of HQ, after different time NF-κB activity assay results and with a significant difference between the control group; compared between the groups, 100 μmol / L of HQ for 72 hours a NF-κB inhibition of the most obvious; and 0.1 μmol / L almost no inhibitory effect of HQ. Conclusion: HQ can inhibit bone marrow stromal cells cultured in vitro transcription factor activation, and with the HQ concentration and the role of time and enhanced speculation may be related to the hematopoietic microenvironment of benzene toxicity. Keywords: stromal cells; in vitro culture; hydroquinone; nuclear transcription factor Hydroquinone Inhibits NF-κB Expression in Human Bone Marrow Stromal Cells In Vitro Abstract This study was aimed to investigate whether hydroquinone (HQ) can inhibit NF-κB expression activated by phorbol myristate acetate (PMA), and to explore the relationship between the mechanism and the hematology toxicity of benzene tentatively. The human bone