Hydroxyacetophenone and the establishment of the Assay.docVIP

 PAGE \* MERGEFORMAT 14 Hydroxyacetophenone and the establishment of the Assay Abstract: Objective was isolated from Artemisia scoparia. Methods silica gel column color Spectrum and Sephadex LH-20 from the chloroform part of Artemisia scoparia isolated pairs of p-hydroxyacetophenone; use DiamonsilTMC18 Column (250mm * 4.6mm, 5μ m), methanol -2% acetic acid solution (30:70) as mobile phase, detection wavelength was 275 nm, Artemisia scoparia of different origin in the content of p-hydroxyacetophenone were determined. The results of p-hydroxyacetophenone in the 0.098 ~ 0.786μ g Fan Wai, a good linear relationship, precision testing, stability testing, repeatability test, plus sample recovery tests were small RSD At 2%, the average recovery was 99.80%. Conclusion The method is accurate, rapid, reproducible and can be used Artemisia scoparia against hydroxyl The determination of Acetophenone. Keywords:: Artemisia scoparia; right-hydroxyacetophenone; separation; determination Artemisia scoparia as’ The 2005 edition of Chinese Pharmacopoeia (1) Artemisia species under the closed set is one of the Compositae Artemisia scoparia Artemisia scoparia Waldst. et Kit. part of the dry ground, with clear hot and humid, the effect of withdrawal of jaundice [1], for the gallbladder to drug withdrawal yellow [2], modern research shows that p-hydroxyacetophenone with a clear choleretic activity of [3], is one of the active ingredient in Artemisia scoparia and Artemisia No existing quality standards determination regarding the contents of the relevant literature [4 ~ 9] has yet to see on the p-B in Artemisia scoparia Determination of in one report. In this study, the use of silica gel column chromatography of the chloroform from the parts of Artemisia scoparia from isolated pairs of hydroxyl Acetophenone, using HPLC determination of the origin Artemisia scoparia 10 pairs of p-hydroxyacetophenone in the content, quality control for Artemisia scoparia And Artemisia her