Hydroquinone in human bone marrow mononuclear cells TOPO expression and its possible mechanism.docVIP

 PAGE \* MERGEFORMAT 15 Hydroquinone in human bone marrow mononuclear cells TOPO expression and its possible mechanism Of: Shiyi Fen Yu Kang, Chen Yi, Ren Hangzhou, Bi to hi, Qian Hong Lan [Abstract] Objective?: To observe the benzene metabolites hydroquinone (hydroquinone, HQ) on human bone marrow mononuclear cells topoisomerase @ (TOPO @) expression, of TOPO @ hematopoietic toxicity caused by the HQ in and its regulatory mechanism. Methods: 50mol / L HQ cultured 10 h in normal human bone marrow mononuclear cells of test group, equal volume of sterile distilled water and cultured 10 h as the control group. Western blot determination of TOPO @ content changes, RT- PCR determination of TOPO @ mRNA expression levels change, ChIP technique was HQ of the bone marrow mononuclear cells TOPO @ promoter histone acetylation, methylation levels. Results: Compared with the control group, 50mol human bone marrow mononuclear cells / L HQ treatment 10 h later, TOPO @ content, TOPO @ mRNA levels were significantly decreased, the difference was significant (P lt;0.01); TOPO @ content decreased along with the TOPO @ promoter of histone H4 acetylation, histone H3K4 methylation significantly reduced the level of (P lt;0.01), histone H3K9 methylation levels (P? lt;0.05), not associated with the level of histone H3 acetylation changes (Pgt; 0.05). Conclusion: HQ can inhibit blood The expression of cell TOPO @; group may be chemically modified proteins of benzene-induced hematopoietic toxicity of metabolites play a role. [Keywords:] hydroquinones; topoisomerase @; histone acetylation; histone methylation; chromatin immunoprecipitation Abstract:? Objective: To observe the effects of hydroquinone? (HQ)? On the expression of topoisomerase @? (TOPO @)? In human bone marrow mononuclear cells, and to explore the possible regulatory mechanism of TOPO @ involving in the toxicity of HQ to hematopoietic cells. Methods: Human bone marrow mononuclear cells was expos