Hydrophilic liquid chromatography triple quadrupole mass spectrometry in the rapid detection of TTX in urine and plasma.docVIP

 PAGE \* MERGEFORMAT 17 Hydrophilic liquid chromatography triple quadrupole mass spectrometry in the rapid detection of TTX in urine and plasma [Abstract] established the rapid detection of TTX in urine and plasma hydrophilic triple quadrupole liquid chromatography mass spectrometry analysis. Samples by acetonitrile precipitation, TSK gel Amide 80 column after the separation of hydrophilic, using electrospray ionization multiple reaction monitoring tandem mass spectrometry (MRM) mode detection, matrix matching the standard external standard. Urine and plasma samples in the linear range of 3 ~ 500 μg / L and 1 ~ 200 μg / L, recoveries were 96% ~ 108% and 100% -105% and relative standard deviation less than 9% and 16% (n = 6), the sample detection limits were 1.0 and 0.3 μg / L (S / N = 3). The method is simple, rapid, sensitive and specific. [Keywords:] hydrophilic liquid chromatography triple quadrupole mass spectrometry, tetrodotoxin, urine, plasma 1 Introduction TTX (Tetrodotoxin, TTX), Department of Pure Japanese scholars Tahara good first from 1909 found in puffer fish, have now isolated a dozen analogues in which the puffer fish, tetrodotoxin as the major toxic components (see Figure 1. Tetrodotoxin is a highly toxic non-protein neurotoxin found in puffer fish in addition, also widely distributed in a variety of vertebrates and invertebrates in vivo. tetrodotoxin into the human body can inhibit the nerve cell membrane Na + permeability thereby blocking nerve impulse conduction, the nerve palsy, resulting in severe inhibition of respiratory deaths [1]. Therefore, a rapid, accurate detection of TTX in urine and plasma of the method has important practical significance. Current, TTX detection methods are mainly biological methods in vivo in mice [2], immunological methods [3], liquid chromatography UV method [4], gas chromatography mass spectrometry [5], post-column derivatization liquid detection [6] and liquid chromatography use of [7 ~ 9],