IgA nephropathy mouse model and identification.docVIP

 PAGE \* MERGEFORMAT 12 IgA nephropathy mouse model and identification [Abstract] Objective To establish a mouse model of IgA nephropathy, IgA nephropathy in clinical studies provide an experimental basis. Methods 30 Kunming mice were divided into normal control group (10), model group (20). The use of oral bovine serum white protein (BSA) combined tail vein injection of staphylococcal enterotoxin B (SEB) of the method of production of IgA nephropathy model in mice. The first 12 weeks to collect fresh urine, qualitative test urine red blood cells under a microscope; eyeball blood, detection of serum creatinine (Scr ), blood urea nitrogen (BUN); light microscopy observation of glomerular mesangial cell and matrix changes; electron microscopy detection of whether the electron dense mesangial deposition; immunofluorescence staining mesangial IgA deposition. Results of 12th week model mice were varying degrees of hematuria; Scr, BUN compared with normal control group was significantly higher (P lt;0.01); light microscope was mesangial cells and matrix hyperplasia; electron microscope, electron dense mesangial deposits; immunofluorescence show a large number of IgA mesangial deposits, and the normal control group were not the performance. Conclusion IgA nephropathy in a mouse model works well, pathology, electron microscopy and clinical parameters and pathology similar to human IgA nephropathy. [Keywords:] IgA nephropathy; model; hematuria; immunofluorescence; mice Abstract: Objective To establish mouse model of IgA nephropathy in order to provide experiment basis for the clinical research of IgA nephropathy. Methods 30 Kunming mice were randomly assigned into the control group (n = 10) and the model group (n = 20). Mouse model of IgA nephropathy was established by the method of oral intake of bovine serum albumin (BSA) together with injection of staphylococcus enterotoxin B (SEB) via the caudal vein. At the end of the 12 weeks, fresh urine was gathered for