Immunohistochemical detection of estrogen and progesterone receptors in breast cancer cells causes coloring of the cytoplasm.docVIP

 PAGE \* MERGEFORMAT 6 Immunohistochemical detection of estrogen and progesterone receptors in breast cancer cells causes coloring of the cytoplasm [Keywords:] immunohistochemistry Immunohistochemical detection of breast cancer tumor cells of estrogen and progesterone receptor (ER, PR is not only in the diagnosis, and treatment for clinical work has also played an important role. Has now been detected in many units as the conventional project. ER, RE expression in the right spot to be in the cell, but in the ER, PR staining process, sometimes coloring the cytoplasm, the issue is confusing. our department since 1993, about 500 cases of breast cancer specimens the ER, PR testing, are combined with the experience and lessons of our work on ER, PR analysis of the causes colored cytoplasm, and to discuss countermeasures. 1 Materials and methods 1.1 antigen retrieval conditions and different antigen retrieval conditions may lead to different staining, and even the phenomenon of colored cytoplasm. In this regard, we have to do antigen retrieval conditions for a number of experiments: (1 enzyme digestion conditions: Let 3 temperature group, the lt;30 , 37 ,gt; 37 , 4 a time groups, 5min, 10min, 20min, 30min group. (2 microwave heat treatment of the antigen retrieval (citrate buffer conditions: temperature set 3 group, that is 80 , 85 , 95 , 3 a time groups, 5min, 10min, 20min time group. 1.2 The first dilution of antibody reagents we have several with the kinds of dilution experiment, found a low degree of anti-dilution were there cytoplasmic staining. An anti-dilution through the higher, the positive strength of the weak and the positive rate is low. As a inappropriate anti-dilution caused by the phenomenon of colored cytoplasm, we have taken the approach is a new advanced anti-reagent, respectively, experiment with different dilutions: Let four dilution groups, dilution and producer, respectively: 10 1:30, 1:50 10, 1:80 to 10, 1:100 10. 1.3 reagent