Injection on the pain must be chronic sciatic nerve ligation model of spinal cord injury in the expression of NR1 mRNA.docVIP

 PAGE \* MERGEFORMAT 8 Injection on the pain must be chronic sciatic nerve ligation model of spinal cord injury in the expression of NR1 mRNA [Abstract] Objective To observe the pain of injection must be chronic sciatic nerve ligation injury model (CCI) rat spinal cord N-methyl-D-aspartate receptor 1 (NR1). Methods 30 male SD rats, randomly divided into normal group, CCI group and the pain will group. measured by RT-PCR, the NR1 expression in rat spinal cord. Results CCI group than in the normal group, the expression of NR1 mRNA in the spinal cord was significantly higher (P lt;0.001) , CCI + PAIN group expression of NR1 mRNA was significantly inhibited (P lt;0.001). Conclusion injection pain must be pain in the spinal cord, its mechanism of inhibition of the expression of NR1 mRNA. [Keywords:] PAIN injection, N-methyl-D-aspartate receptor 1, RT-PCR, animal models Abstract: Objective To study the effect of Tongbiding injection on expression of N-methyl-D-aspartate (NMDA) receptor 1 mRNA in the spinal cord of chronic constriction injury (CCI) rats. Methods Thirty male Spragru-Dawley rats were randomly divided into control group (group A), CCI group (group B) and Tongbiding group (group C), ten rat for eah group. The changes in NR1 mRNA expression in the spinal cord was detected by reverse transcription-polymerase chain reaction (RT-PCR ) techniques. Results The expression of NR1 mRNA in the spinal cord was minimal in control group, CCI induced significant increase in the expression of NR1 mRNA in the spinal cord and Tongbiding significantly inhibited the increase in NR1 mRNA expression. There was significant difference between CCI and Tongbiding group (P lt;0.001). Conclusions Inhibition NR1 mRNA expression in the CCI rats spinal cord may contribute to analgesic mechanisms of Tongbiding in the spinal dorsal horn. Keywords:: Tongbiding injection, NR1 mRNA, RT-PCR; animal model PAIN injection of 4 Chinese herbs (the active ingredient or part), with