Keratinocytes intracellular antibacterial activity of Staphylococcus aureus.docVIP

 PAGE \* MERGEFORMAT 11 Keratinocytes intracellular antibacterial activity of Staphylococcus aureus Study: Rock Gengkuai Zhen Jiang Liu Ning Wang Boyao Wu Qi Xian Huang [Abstract] Objective: Staphylococcus aureus in human keratinocyte HaCaT cells to survive the dynamic changes, understanding of S. aureus with the skin between keratinocytes. Methods: The standard strain ATCC25923 Staphylococcus aureus invasion HaCaT cells were in the bacteria into the cells 4,24,48,72 h after cell lysis and release of live intracellular bacteria colony count method using plate count of intracellular viable. Results: Staphylococcus aureus ATCC25923 strain into HaCaT 24h cells have some growth, but the experiment 48h decreased the number of viable cells. protein kinase C activator PMA and the adenylyl cyclase activator FSK HaCaT cells can promote the clearance of intracellular bacteria. Conclusion: The removal of skin keratinocytes into cells of Staphylococcus aureus, the skin may be a natural immune defense mechanisms, and PMA and FSK enhanced cell antibacterial effect, suggesting keratinocyte antimicrobial activity and NADPH oxidase-related. [Keywords:] keratinocytes; Staphylococcus aureus; antimicrobial Is the body’s natural immune system against invading pathogens, the first line of defense, it can be more targeted immune system to produce the specific response before, first to eliminate or mitigate the consequences of the invasion of pathogens. Innate immunity by physical, chemical and biological Science barriers, block or destroy invading pathogens, while reducing tissue damage and cell necrosis, containing pathogens may cause changes in the pathophysiology. epithelial cells are an important component of innate immunity, recent studies have shown that epithelial cells through direct contact with the bacteria , phagocytic uptake of bacteria or epithelial cells, epithelial cells have strong antibacterial ability. Keratinocytes in the skin immune defense and infl