Liposomal docetaxel pharmacokinetics study in rats.docVIP

 PAGE \* MERGEFORMAT 10 Liposomal docetaxel pharmacokinetics study in rats Author: Rui Liu Ying, Wang suming, Qing-Guo Song [Abstract] Objective To explore docetaxel liposomes in vivo metabolism in rats. Methods to optimize the chromatographic conditions in order to extract concentration method with biological samples, internal standard Determination of drug content in the sample; SD rats were determined after intravenous administration in vivo drug concentration set point in time, with version 2.0 software analysis of drugs in vivo kinetics of DAS parameters. Results The optimized chromatographic conditions, in the 0.05 ~ 25 μ g / ml concentration range, docetaxel linear relationship was good. Drug in vivo process for two-compartment model, the main mean of pharmacokinetic parameters: distribution half-life (T1/2α ) of 2.374 min; elimination half-life (T1/2β ) of 299.038 min; clearance rate (CL) of 0.026 L / (min * mg); apparent volume of distribution (V1) of 0.219 L / kg. Conclusion The study for the development of docetaxel in vivo kinetics of liposome to provide references. [Keywords:] docetaxel; Liposome; pharmacokinetics; rat Abstract: ObjectiveTo establish an HPLC method for in vivio docetaxel determination and do some research on pharmacokinetics of docetaxel liposomes in rats. MethodsHPLC method was optimized and samples were concentrated via ether extraction and determined with inner-standard method. Docetaxel liposome was given intravenously at 10 mg / kg to SD rats and blood sample was taken at chosen time interval and docetaxel conentration was determined, according to which pharmacokinetic parameters were gained via DAS 2.0.ResultsThe chosen mobile phase was methonol: water (45:55) and wavelength was 233nm. Within the range of 0.05 ~ 25 μ g / ml there was an excellent linear correlation and the regression equation of peak area ratio to concentration was Y = 0.396 8 X 0.680 8 (r = 0.999 4). The pharmacokinetic result revealed it a double-depar