Lipstatin screening high-yielding strains.docVIP

 PAGE \* MERGEFORMAT 11 Lipstatin screening high-yielding strains [Abstract] in order to poison three prime Streptomyces XC-lp-2 variable strain OT-3 as the starting strain by UV mutagenesis and microwave complex processing and the plate containing soybean oil soybean oil-resistant mutant targeted screening, access to high-yield mutant XC-lp-69, its lipstatin fermentation titer reached 911μ g/ml, compared with the starting strain OT-3 increased 71.6%. Passage results show that mutant XC-lp-69 high-yielding stable genetic characteristics. [Keywords:] Lipstatin; strain breeding; mutagenesis; poison three prime Streptomyces ABSTRACT After Stretomyces toxytricini OT-3 was treated by UV and microwave irradiation, the soybean oil tolerant mutants were screened and a high-titre strain XC-lp-69 was obtained. The productivity of strain XC-lp-69 reached 911 μ g / ml , which is 71.6% more than that of the starting stain OT-3. The subculture experiments indicated that the hereditary character of high productivity of strain XC-lp-69 is stable. KEY WORDS Lipstatin; Strain breeding; Induced mutation; Streptomyces toxytricini Received date :2006-11-29 Author: Han Junru, female, born in 1966, female, Master. Research Interests: Bio-pharmaceuticals. Lipstatin (Lipp Secretary, statins) is a poisonous three prime Streptomyces (Stretomyces toxytricini) metabolites, can selectively inhibit the gastrointestinal tract in the pancreatic lipase activity, reduce fat breakdown and absorption of its tetrahydro derivatives orlistat (orlistat) has been developed by Roche for weight loss medication (brand name: Xenical, Xenical) [1,2], is the only one listed as a non-central nervous system of drug treatment of obesity [3]. Therefore, the fermentation production of lipstatin concern. Lipstatin fermentation to improve the level of production mainly depends on strain selection and fermentation processes (culture medium and culture conditions) the optimization of these two complementary a