Lipoxygenase catalyzed double high γ linolenic acid lipid free radical oxidation of the carbon structure confirmed.docVIP

 PAGE \* MERGEFORMAT 18 Lipoxygenase catalyzed double high γ linolenic acid lipid free radical oxidation of the carbon structure confirmed Study: Xiao Ying Qing Feng Steven Y. Qian Kun-Yi Ni [Abstract] liquid chromatography electron spin resonance spectroscopy (LC / ESR) combined with technology, liquid chromatography mass spectrometry (LC / MS) combined with combined with spin trapping technique to study the lipoxygenase (LOX) catalyzed double high γ linolenic acid (DGLA) produced during lipid oxidation of carbon radicals. to @ [4-pyridyl 1 O] N N-tert-butyl ketone (POBN) as spin trapping agent, the reaction mixture in the LOX DGLA in the formation of free radicals and spin adducts of carbon, according to the adducts in LC / UV / ESR and LC / MS retention time corresponding to determine the molecular weight adducts, and further fragmentation under way to determine the adduct its structure. The results show that DGLA in the LOX catalyzed lipid oxidation of carbon radicals include C7H13O2, · C10H17O2 and C5H11, oxygen free radicals from DGLA fat (8, 11, 15 LO ·) of β cleavage. The result is conducive to further research DGLA in vivo lipid peroxidation process and the carbon produced during the physiological role of free radicals. [Keywords:] double high γ linolenic acid, lipid peroxidation, spin trapping, liquid chromatography combined with electron spin resonance spectroscopy, liquid chromatography mass spectrometry 1 Introduction Double high γ linolenic acid (DGLA, 20:3, ω 6) in the body ω 6 polyunsaturated fatty acid metabolism intermediates [1]. Linoleic acid (LA, 18:2, ω 6) in the Δ6 desaturase formed by dehydrogenation under the action of γ linolenic acid (GLA, 18:3, ω 6), GLA in the extension under the action of the enzyme Δ5 desaturase DGLA.DGLA in by dehydrogenation under the action of arachidonic acid can be converted to (AA , 20:4, ω 6). DGLA, AA and eicosapentaenoic acid (EPA, 20:5, ω 3 is the synthesis of prostaglandin (PG) and thro