Liposome-mediated VEGFC antisense oligonucleotides on lung cancer lymphangiogenesis of.docVIP

 PAGE \* MERGEFORMAT 13 Liposome-mediated VEGFC antisense oligonucleotides on lung cancer lymphangiogenesis of Of: Dingcheng Zhi Yue Guo Xufeng Qian Xu Zhonghua Xu Zhongheng [Abstract] Objective To observe the liposome-mediated vascular endothelial growth factor C (VEGF C) antisense oligonucleotides on lung tumor model in nude mice growing impact of lymphatic vessel formation. Methods BALB / C mice skin cancer Model 30, were randomly divided into control group, phosphate buffer (PBS group), sense oligonucleotide group (SODN group) and antisense oligonucleotide treatment group (AODN group). inoculated with human lung adenocarcinoma cell line within 24 h after A549, respectively PBS, SODN and AODN subcutaneous treatment, the tumor growth in mice .4 w, the animals were sacrificed and specimens from tumor specimens, using RT PCR, Western blot and immunohistochemistry Detection of VEGF C antisense oligonucleotide on growing tumors and VEGF C expression levels and the impact of lymphatic vessel formation. Results AODN group VEGF C mRNA and protein expression was reduced (4.61 + -1.57), compared with the control group was significant ( P lt;0.05), planting density of intratumoral lymphatic vessel (3.2 + -1.7) was significantly lower than the control group (P lt;0.01). Conclusion liposome-mediated antisense VEGF C can significantly reduce lung cancer subcutaneous tumor model in nude mice the expression of VEGF C and its lymphangiogenesis with strong inhibition. [Keywords:] lung cancer, vascular endothelial growth factor C, gene therapy Non-small cell lung cancer (NSCLC) accounts for about 80% of lung cancer, along with the comprehensive treatment and individual treatment deepening the understanding and promotion of, NSCLC treatment has been greatly improved, but still lower than the current 5-year survival rate of 15% (1). is generally believed that invasive and metastatic tumor cells is to determine the tumor type and the leading cause of death in patients