Lishui Zhejiang Pseudomonas aeruginosa genes encoding aminoglycoside-modifying enzymes.docVIP

 PAGE \* MERGEFORMAT 12 Lishui Zhejiang Pseudomonas aeruginosa genes encoding aminoglycoside-modifying enzymes 【Abstract】 Objective To understand the clinical isolates of Pseudomonas aeruginosa in the presence of aminoglycoside modifying enzyme gene encoding the situation. Methods of 40 clinical isolates of Pseudomonas aeruginosa by polymerase chain reaction (PCR) detection of genes [aac (3)  II, aac (6 ‘)  I, aac (6’)  II, ant (3 “ )  I, ant (2 “)  I]. Results Of the 40 strains of aac (6 ‘)  I-positive 18 (45.0%), ant (2 “)  I-positive 21 (52.5%), and the remaining genes were negative. Conclusions clinical isolates of Pseudomonas aeruginosa in Lishui aminoglycoside-modifying enzyme encoding gene carrying a high rate. Keywords: Pseudomonas aeruginosa gene encoding aminoglycoside-modifying enzyme PCR China-resistant pathogens in hospitals monitoring network statistics [1], in recent years, Pseudomonas aeruginosa (Pseudomonas aeruginosa, Pa) is a common clinical conditions, one of the pathogenic bacteria. Pa right, including β  lactam and aminoglycoside antibiotics commonly used there, including multi-drug resistance, and lead to serious hospital infections caused great difficulty for clinical treatment. Pa through the acquisition of aminoglycoside-modifying enzyme aminoglycoside antibiotics and resistance to passivation [2]. In order to understand Pa nosocomial infection in our hospital isolates of aminoglycoside-modifying enzymes in the gene encoding the existence condition of 40 Pa was aac (3)  II, aac (6 ‘)  I, aac (6’)  II, ant (3 “)  I and ant (2”)  I and other 5 kinds of aminoglycoside-modifying enzyme encoded genetic testing studies, are reported as follows. 1 Materials and methods 1.1 Strain Source 40 Pa were isolated from the Lishui City, Zhejiang Province People’s Hospital in December 2004 ~ December 2005-patient clinical specimens. With the Bio  Merieux’s ATB system, identification of bacteria.