Maodongqing roots lignan glycoside extraction and purification of Identification.docVIP

 PAGE \* MERGEFORMAT 7 Maodongqing roots lignan glycoside extraction and purification of Identification Authors: Zhou mid-stream, Zou Jie-Ming, Li-Sheng Wang, Zeng-li, YIN Wen-qing [Abstract] Objective To study the total Maodongqing lignans glycosides optimal extraction process, and total lignan glycosides were purified identification. Methods orthogonal experiment, examining ethanol concentration, extraction temperature, solid-liquid ratio, and extraction time on the four factors, the impact of technology; the use of D101 macroporous resin separation and purification Maodongqing total lignan glycosides. The results identified a total Lignans Maodongqing wooden body with the best extraction of sugar as follows: extraction time 1.5 h, extraction temperature 80 ℃ , ethanol concentration 70%, solid-liquid ratio 1:5; purified total lignans glycosides were confirmed by IR and tortoside A preliminary identification of lignans reference substance have the basic structural characteristics of the mother nucleus. Conclusion The optimum extraction was optimized conditions and purification of total lignan glycosides method for further process development there is a good reference value. [Keywords:] Maodongqing lignan glycosides Extraction, Purification and Identification Abstract: ObjectiveTo study the optimum extraction process for the total lignans from Pubescent Holly root, and lignans was purified and characterized. MethodsBy orthogonal design, the effects of concentration of ethanol, temperature, ratio of solid to liquid and time of extraction on the total lignans extraction rate were investigated.The total lignans were separated and purified by D-101 porous resin absorption. ResultsThe optimum extraction condition was at temperature of 80 ℃ , concentration of alcohol was 70%, solid / lipuid ratio was 1:5 and extraction time was 1.5 h. Its structure was characterized by IR spectrum, the result showed that both the total lignans and tortoside A had the same constru